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Why Is D2O Used In Ir Spectroscopy? [Solved]

Well, swapping out H2O for D2O in infrared experiments is common, but getting rid of water from protein samples can be a real pain. It’s tricky and time-consuming, and if you don’t do it right you could end up with denatured proteins or even lose some of your sample. Dialysis isn’t much help either when it comes to suspensions and sols.